Cloning and expression of H.pylori ahpC in prokaryotic expression vector
- VernacularTitle:幽门螺杆菌ahpC基因的克隆与原核表达
- Author:
Yanqing LI
;
Guangcai DUAN
;
Chunhua SONG
;
Jianguang ZHANG
;
Shuling WANG
;
Weidong ZHANG
;
Yuanlin XI
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
ahpC gene;
cloning;
gene expression
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):47-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a prokaryotic expression system of ahpC gene of Helicobacter pylori. Methods The ahpC gene was amplified from Hp chromosomal DNA by PCR technique and cloned into the expression vector pET-30a. The recombinant vector pET30a-ahpC was identified by DNA sequencing and transformed to E.coli BL21 (DE3) for expression under induction by IPTG. The expression product was analyzed by SDS-PAGE. Results PCR product showed that ahpC gene consisted of 594bp. The gene fragment that was inserted into the recombinant vector was identified to GenBank for 99%. SDS-PAGE showed that the induced protein was expressed highly in the host bacterium. Conclusion A prokaryotic high-expression system for ahpC gene has been successfully constructed. It can highly express r-AhpC protein in E.coli.
