Determination of sulfydryl-containing drugs by reverse flow injection chemiluminescence analysis
- VernacularTitle:反相流动注射化学发光法测定含巯基药物的含量
- Author:
Guangbin ZHANG
;
Xianmin CHEN
;
Haiyan LI
;
Hongling YU
;
Sijuan SUN
;
Yuhai TANG
- Publication Type:Journal Article
- Keywords:
flow injection;
chemiluminescence;
sulfydryl-containing drug
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):125-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid, accurate and sensitive chemiluminescence method for determining sulfydryl-containing drugs. Methods In sulfuric acid solution, glyoxal could be oxidized by potassium permanganate, and weak chemiluminescence could be observed. Chemiluminescence signal could be enhanced in the presence of sulfydryl-containing drugs. Thus, on this basis we established a new method of determining the concentration of sulfydryl-containing drugs with flow injection chemiluminescence analysis. Results Under the optimized conditions, the linear range of methimazole, captopril and acetylcysteine was 1.0×10~(-8)- 5.0×10~(-6), 7.0×10~(-8)-1.0×10~(-6) and 3.0×10~(-8)-1.0×10~(-6)g/mL, respectively. The limit of detection of methimazole, captopril and acetylcysteine was 1.0, 3.9 and 3.7ng/mL, respectively. Conclusion The method was successfully applied to determine the three drugs that contain sulfydryl. Compared with the results of pharmacopeia methods, the results we obtained were satisfactory.
