Construction of eukaryotic expression vector of wtp53/junB fusion gene
- VernacularTitle:野生型p53/junB融合基因真核表达载体的构建
- Author:
Cheng GUO
;
Lei ZHANG
;
Qingguang LIU
;
Tao SONG
;
Xue YANG
;
Xin ZHENG
;
Yingmin YAO
- Publication Type:Journal Article
- Keywords:
wtp53;
junB;
HepG2;
fusion gene;
hepatocellular carcinoma
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):41-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct wtp53/junB fusion gene and its eukaryotic expression vector in order to provide the basis for further application of polygene union therapy in hepatocellular carcinoma. Methods Polymerase chain reaction (PCR), reverse transcription-PCR (RT-PCR) and gene recombination techniques were used to construct the eukaryotic vector of pEGFP-C1-wtp53/junB fusion gene, which carries the enhanced green fluorescent protein (EGFP). The transfection of pEGFP-C1-wtp53/junB in hepatoma HepG2 cells was detected by the location of green fluorescence. Results The DNA sequence of wtp53/junB fusion gene was successfully cloned into the pEGFP-C1 plasmid and the sequence was the same as what we expected. Green fluorescence located on cell nucleus proved that pEGFP-C1-wtp53/junB was transfected into HepG2 cell line successfully. Conclusion We successfully constructed the eukaryotic vector of pEGFP-C1-wtp53/junB fusion gene, which carries the EGFP, and transfects it into human hepatoma cell nucleus. It may lay the basis for studying the synergetic effect of wtp53 and junB in hepatocellular carcinoma.
