Effect of endoplasmic reticulum stress in renal damage caused by hyperlipidemia
- VernacularTitle:内质网应激在高脂血症引起肾脏损害中的作用
- Author:
Xiaodong CHANG
;
Hua GAN
;
Xiaogang DU
;
Lixue CHEN
- Publication Type:Journal Article
- Keywords:
hyperlipidemia;
endoplasmic reticulum stress;
glucose-regulated protein 78;
CHOP
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):79-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of endoplasmic reticulum stress in renal damage caused by hyperlipidemia. Methods Forty male Wistar rats were randomly divided into two groups: normal control group (NC, n=20) and high-fat group (HF, n=20). Rats in NC group were fed with normal diet while those in HF group were fed with high-fat diet. Five rats in each group were randomly chosen in week 4, 8, 12 and 18. Serum lipid, urine protein in 24 hours and the pathological changes of renal tissues were observed; the apoptosis of renal cells was detected by TUNEL staining; the expression of GRP78 protein in the kidney was examined by immunohistochemistry. The expression of GRP78 mRNA and CHOP mRNA was examined by RT-PCR. Results Compared with those in NC group, serum lipid as well as the expression of GRP78 mRNA and protein in the kidney were increased in week 4, 8, 12 and 18 in HF group(P<0.05). In contrast, urine protein in 24 hours, the apoptosis index of renal cells and the expression of CHOP mRNA were increased in week 8, 12 and 18 (P<0.05). Conclusion CHOP pathway of endoplasmic reticulum stress is involved in renal damage caused by hyperlipidemia.
