Qualitative and quantitative study on the apoptosis of cervical cancer cells treated by tetrandrine
- VernacularTitle:粉防己碱诱导宫颈癌细胞凋亡的定性定量研究
- Author:
Kexiu ZHU
;
Bin LI
;
Zhuo DENG
;
Caiping YUAN
;
Jianning MU
;
Xiaobing HAN
- Publication Type:Journal Article
- Keywords:
tetrandrine;
cervical cancer;
apoptosis
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(1):102-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of tetrandrine on apoptosis of HeLa cervical cancer cells qualitatively and quantitatively. Methods We measured tetrandrine-induced inhibition of HeLa cell proliferation at different concentrations and time points by MTT assay. The rate of Hela cell apoptosis induced by tetrandrine was detected by flow cytometer and confocal laser scanning microscope (CLSM). Results Tetrandrine inhibited the proliferation of HeLa cells in dosage- and time-dependent manners. Flow cytometry showed that the apoptosis rate was (51.8±0.97)% at the concentration of 15μmol/L, which was significantly higher than that in the control group (24.3±1.23)% (P<0.05). The cells treated with tetrandrine showed typical apoptotic morphology under CLSM. Conclusion Tetrandrine can inhibit proliferation and induce apoptosis of HeLa cervical cancer cells.
