Effect of tea polyphenols on oxidative damage and apoptosis in human bronchial epithelial cells induced by low-dose cigarette smoke condensate
10.3969/j.issn.1672-7347.2010.02.006
- VernacularTitle:茶多酚对低剂量烟草悬凝物诱导人支气管上皮细胞氧化损伤及凋亡的影响
- Author:
Chunhua QING
;
Ping CHEN
;
Xudong XIANG
- Publication Type:Journal Article
- Keywords:
tea polyphenols;
cigarette smoke condensate;
human bronchial epithelial cells;
reactive oxygen species;
apoptosis
- From:
Journal of Central South University(Medical Sciences)
2010;35(2):123-128
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of tea polyphenols on oxidative damage and (apoptosis) in human bronchial epithelial cells induced by low-dose cigarette smoke condensate (CSC).Methods We prepared CSC. 3-(4,5-dimethyl thiazoly) 2,5-diphenyl-tetrazoliun bromide (MTT) assay was used to determine the growth of cultured human bronchial epithelial cells (HBE135-E6E7). Fluorescent-chemiluminescent analyzer was used to measure cell reactive oxygen species (ROS) level. DNA ladder method was used to detect HBE135-E6E7 apoptosis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect Bcl-2 and Bax mRNA expression.Results Concentration of intracellular ROS in the CSC group and CSC + TP group was significantly higher than that in the control group (P＜0.01); concentration of intracellular ROS in the CSC + TP group was significantly lower than that in the CSC group (P＜0.01). Apparent DNA breakage of the tail belt appeared in the CSC Group,while only a small amount of DNA breakage of the tail belt appeared in the CSC + TP group. Compared with the control group, Bcl-2 mRNA expression was reduced and Bax mRNA expression was increased in the CSC group (all P＜0.01). Compared with the CSC group, Bcl-2 mRNA expression was increased and Bax mRNA expression was reduced in the CSC+TP group (all P＜0.01). Ratio of Bcl-2 mRNA/ Bax mRNA in the CSC group and CSC+TP group was significantly lower than that in the control group (all P＜0.01).Conclusion TP can antagonize CSC-induced airway epithelial cell apoptosis through the effective removal of ROS, promoting Bcl-2 mRNA expression and inhibiting the expression of Bax mRNA.
