Effect of adenovirus-mediated IL-24 gene on the topoisomeraseⅡα and Caspase-3 expression in glioma cell line U251
10.3969/j.issn.0529-1356.2010.01.010
- VernacularTitle:腺病毒介导的IL-24基因对胶质瘤细胞系U251拓扑异构酶Ⅱα及Caspase-3表达的影响
- Author:
Shuo HAN
;
Huixian CUI
;
Wenling LI
;
Junxia ZHAO
;
Cuili CAO
;
Yunli YAN
- Publication Type:Journal Article
- Keywords:
IL-24 gene;
TopoisommeraseⅡα;
Caspase-3;
Glioma;
Western blotting;
Flow cytometry;
Glioma cell line U251
- From:
Acta Anatomica Sinica
2010;41(1):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective The present study is to investigate IL-24 gene(Ad5F35-hIL-24) effect on the topoisommeraseⅡα(topoⅡα) and Caspase-3 expression in glioma cell line U251. Methods After transfected the U251 glioma cells with the Ad5F35-hIL-24, the methyl thiazolyl tetrazolium (MTT) was used to analyse the inhibition rate of Ad5F35-hIL-24 on the cells. Hoechst 33258 fluorescent staining and flow cytometric assay were used to detect apoptosis. The immunohistochemistry assay was used to detect topoⅡα expression, and Western blotting was applied to detect the protein expression of topoⅡα and caspase-3. Transwell experiment was used to test the invasiveness of the cells. Results It was found that the Ad5F35-hIL-24 could inhibit U251 cell proliferation and induce apoptosis in a dose dependent manner compared with the control groups. It showed that Ad5F35-hIL-24 could inhibit topoⅡα expression reveled by immunohistochemistry and Westeren blotting, while it increased caspase-3 protein expression. The Transwell experiment showed that the Ad5F35-hIL-24 could reduce the invasiveness of the U251 glioma cells.Conclusion The exogenous IL-24 gene can inhibit the cell proliferation and induce apoptosis of U251 glioma cells. The topoⅡα and Caspase-3 are the important molecular targets of the IL-24 gene. These results may give support for the IL-24 gene usage in clinical treatment for glioma patients.
