Clinical significance of thrombospondin 4 expression in gastric carcinoma
10.3969/j.issn.0529-1356.2010.01.013
- VernacularTitle:血小板反应素-4在胃癌中的表达及其临床意义
- Author:
Xiandong LIN
;
Yuanlin QI
;
Shuqin CHEN
;
Yang TANG
;
Jianyin LIN
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
Thrombospondin-4;
Microvessel density;
Matrix metalloproteinase-9;
Immunohistochemistry;
RT-PCR;
Human
- From:
Acta Anatomica Sinica
2010;41(1):65-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to measure quantitatively the TSP-4 mRNA expression and its significance in gastric carcinoma was evaluated by correlating its expression with clinicopathological features, microvessel density(MVD) and MMP-9.Methods Eighty-two patients with gastric carcinoma were recruited in this study. TSP-4 mRNA expression in gastric carcinoma and adjacent tissue was detected by real-time PCR. CD34 and MMP-9 protein expression were examined by immunohistochemistry. MVD was determined according to CD34-positive tubular structures. Results The expression level of TSP-4mRNA in gastric carcinoma was obviously higher than those of adjacent tissue(P=0.03) and it was associated with tumor size, histological type, lymph node metastasis, MVD and MMP-9(P=0.002, P=0.031, P=0.014,r＝0.67 P<0.01, P=0.008),but not sex, age and depth of invasion. (P=0.53,P=0.57,P=0.15).Conclusion TSP-4 may play an important role in occurrence and progression of gastric carcinoma and that the effect of TSP-4 on tumor growth and metastasis may be exerted through regulation of angiogenesis and MMP-9 expression in gastric carcinoma.
