Expression of NF-κB and regulated upon activation normal T cell expressed and secreted chemokine in experimental abscending aortic aneurysm rat model
10.3969/j.issn.0529-1356.2010.01.015
- VernacularTitle:NF-κB及调节活化正常T细胞表达和趋化因子在升主动脉瘤形成中的表达
- Author:
Wanwei GUO
;
Shuling BAI
;
Jun WANG
;
Jun FAN
;
Xiaohong TIANG
- Publication Type:Journal Article
- Keywords:
NF-κB;
RANTES;
Ascending aortic aneurysm;
RT-PCR;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2010;41(1):75-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of nuclear factor kappaB(NF-κB) and regulated upon activation normal T cell expressed and secreted chemokine(RANTES) during the formatiom of ascending aortic aneurysm. Methods Forty Wistar rats were randomly divided into the control group(n=20) and the experimental group(n=20).The rat models were made by ligating the ascending aorta. The ascending aortas were taken after ligation for 3months. Immunohistochemistry staining was performed to detect the protein expression of NF-κB and RANTES. The expression of NF-κB and RANTES mRNA were detected by RT-PCR. Results Immunohistochemisry staining results showed NF-κB and RANTES expression significantly increased in aneurysm, while there was a little positive staining in the control group. RT-PCR results indicated that the expression levels of NF-κB and RANTES in the aneurysm were stonger than that of the control group. The expression of NF-κB and RANTES mRNA were remarkably correlated. Conclusion The expression of NF-κB and RANTES in ascendin aortic aneurysm are stronger than that in the control. NF-κB and RANTES may contribute to the pathogenesis of the ascending aortic aneurysm.
