Expression of endothelin-1 in cerebral vessels of scalded rats
10.3969/j.issn.0529-1356.2010.01.020
- VernacularTitle:内皮素-1在烫伤大鼠脑血管损伤中的表达
- Author:
Xuequn LIN
;
Yucheng DAI
;
Gaochun ZHU
;
Lidan WAN
- Publication Type:Journal Article
- Keywords:
Endothelin-1;
Scald;
Cerebral artery;
Western blotting;
RT-PCR;
Radioimmunoassay;
Rat
- From:
Acta Anatomica Sinica
2010;41(1):104-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and effects of endothelin-1 (ET-1) in cerebral vessels of scalded rats. MethodHistological method was used to observe the alteration of histological structure of basilar artery in 8 scalded rats. Reverse transcription polymerase chain reaction(RT-PCR) was performed to examine expression of ET-1 mRNA;Western blotting to analyse expression of endothelin-1;radioimmunoassay to measure ET-1 levels;R3espectively, of cerebral basilar artery in 48 scalded rats. ResultVascular pathohistological changes were observed in cerebral basilar artery after rats were scalded;ET-1, ET-1 mRNA expression and ET-1 levels of basilar artery in scald 6 hours, 12 hours and 24 hours group were obviously higher than that of control group. Conclusion Scald may cause an increases of ET-1 expression in basilar artery, and elevated ET-1 expression may relate to the pathogenesis of cerebral vascular injury in scalded rats.
