Significance and expression of S100A9 and NMP238 in cervical carcinoma tissues with different concurrent chemoradiotherapy sensitivities
10.3969/j.issn.1672-7347.2010.01.007
- VernacularTitle:S100A9和NMP238在同时放化疗敏感性不同宫颈癌中的表达及意义
- Author:
Hong ZHU
;
Shan ZENG
;
Liang ZENG
;
Xuewei WANG
;
Zhiqiang XIAO
- Publication Type:Journal Article
- Keywords:
cervical carcinoma;
concurrent chemoradiotherapy;
proteomics;
immunohistochemistry
- From:
Journal of Central South University(Medical Sciences)
2010;35(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the significance and expression of S100A9 and NMP238 in cervical carcinoma with different concurrent chemoradiotherapy sensitivities. Methods Fresh carcinoma tissues were collected from untreated cervical carcinoma patients and preserved at -80 ℃. The tissues were classified into 2 groups:a high sensitivity group (HS) and a low sensitivity group(LS) according to their response to concurrent chemoradiotherapy. Protein was separated by 2-dimensional gel electrophoresis (2-DE). Peptide mass fingerprintings (PMF) were acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and the proteins were identified by data searching in the Mascot-database. Differential expressed proteins were assayed by Western blot and immunohistochemistry.Results Most of the gels were clear and were successfully and reproductively analyzed. Intensity and rate of S100A9 expression were higher in the HS group than in the LS group,and those of NMP238 expression were higher in the LS group than in the HS group. Conclusion S100A9 and NMP238 expression is associated with concurrent chemoradiotherapy sensitivity in cervical carcinoma.
