Determination of Cyromazine and Dicyclanil Residues on Greasy Wool by High Performance Liquid Chromatography
- VernacularTitle:高效液相色谱法测定含脂羊毛中灭蝇胺和环虫腈
- Author:
Yuanmu FAN
;
Xuejun YU
;
Xiaojun GU
;
Juyi YIN
;
Yajun QIU
;
Shubing CHEN
;
Jie CHEN
;
Jia ZHAN
;
Xiaoyu HE
;
Jun CHEN
;
Shaotang HUANG
- Publication Type:Journal Article
- Keywords:
Cyromazine;
Dicyclanil;
Greasy wool;
Pesticide residue;
High performance liquid chromatogra phy;
High performance liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2010;38(1):113-116
- CountryChina
- Language:Chinese
-
Abstract:
A method for the determination of cyromazine and dicyclanil residues on greasy wool was developed with HPLC and confirmed with HPLC-MS/MS.The cyromazine and dicyclanil residues on greasy wool were extracted with 1% trichloroacetic acid solution with ultrasonic, and cleaned up by MCX SPE column.The HPLC separation was performed on a Hypersil NH_2 using water-acetonitrile (V/V) as the mobile phase with gradient elution and detected at the wavelength of 214 nm.The corroboration method of HPLC-MS/MS was used with electro-spray ionization of positive ion mode.The [ M + H ]~+ and characteristic ions of dicyclanil were m/z 191.0, 150.0 and 163.0, and cyromazine were m/z 167.0, 85.0 and 125.0.The linear ranges of cyromazine and dicyclanil were 0.05-5.0 mg/L.There were good linear relationships between the peak area and concentration in the linear range.The correlation coefficient was 0.9999.The detection limit of cyroma zine was 0.02 mg/kg, and dicyclanil was 0.01 mg/kg.The average recoveries of cyromazine and the dicycla nil were 95.0%-99.9% and 83.6%-92.2%, respectively.
