Detection of Staphylococcus Aureus in Different Liquid Medium Using Wireless Magnetoelastic Sensor
- VernacularTitle:磁弹性无线传感器检测不同液体介质中的金黄色葡萄球菌
- Author:
Sijing HUANG
;
Yijie WANG
;
Qingyun CAI
;
Jiande FANG
- Publication Type:Journal Article
- Keywords:
Magnetoelastic wireless sensor;
Staphylococcus aureus(S. aureus);
Culture medium;
Milk
- From:
Chinese Journal of Analytical Chemistry
2010;38(1):105-108
- CountryChina
- Language:Chinese
-
Abstract:
A magnetoelastic Staphylococcus aureus (S.aureus) wireless sensor was fabricated to detect S.aureus in different liquid medium.After 0.2 mL bacteria with proper concentration was taken into the tes ting cuvette containing 2 mL liquid medium, the resonance frequency of magnetoelastic sensor changed with the bacteria growth.Via changing the concentration of beef extract and peptone, sensor had the most frequen cy response in culture medium CM2-2 incubated with 2 × 10~5 cells/mL S.aureus.The results indicated that this sensor can be used to determine S.aureus concentrations of 3 × 10~3 to 2×10~7 cells/mL in culture medium and 10~4 to 10~7 cells/mL in milk, with the detection limits of 10~3 cells/mL and 104 cells/mL, respectively.The sensor frequency shift was correlated to the logarithmic value of the S.aureus concentration, and the correlation coefficient was 0.98 in milk and 0.99 in culture medium.
