Obtaining of union expression virus-like particle of PPV VP2 and PCV2 ORF2 and its immunogenicity
- VernacularTitle:联合表达PCV2 ORF2和PPV VP2基因病毒样颗粒获得及免疫原性
- Author:
Zhiwen XU
;
Wanzhu GUO
;
Yang CHEN
;
Ling ZHU
;
Yanling CHEN
;
Yin WANG
- Publication Type:Journal Article
- Keywords:
virus-like particles(VLPs);
porcine parvovirus (PPV);
porcine circovirus 2 (PCV2);
vaccine
- From:
Chinese Journal of Veterinary Science
2009;29(7):821-825
- CountryChina
- Language:Chinese
-
Abstract:
The amplified VP2 gene (PPV strain SC-1) and PCV20RF2 gene were inserted into the eukaryotic expression vector pPI-2.EGFP.Then the recombinant plasmid named pPI-2.EGFP.VP2.ORF2 was obtained.Mediated by liposome,the recombinant plasmid was transfected into Veto cells and expressed.Using immunofluorescence assay,the fluorescence of expression products were first detected at 20 h after transfection and peaked 36 h.Under electronmicroscope,virus-like particles (VLPs) can be observed in the transfected cells.To confirm the obtained VLPs to be recombinant particles,piglets were immunized using purified VLPs.The dynamic variation of blood T lymphocytes and serum antibody level of PPV and PCV2 were measured.The results showed that the ratio of CD3+,CD4+ T lymphocyte in peripheral blood lymphocyte of immunized piglets raised in a certain degree,the number of CD8+ T lymphocytes fell at 7-14 d after immunization,and then raised.Relatively high level of PP-V,PCV2 specific antibody could be detected.This indicated that the expression of recombinant plasmid pPI-2.EGFP.VP2.ORF2 was successful,the virus-like particles were formed and showed favourable immunogenicity.
