Screening of tumor mark for the lung cancer by using peptide ZS-6
- VernacularTitle:利用肺癌特异性结合多肽ZS-6筛选肺癌相关标志物
- Author:
Wenbin FENG
;
Xuediao PAN
;
Jie ZHOU
;
Lei SHI
;
Junqiang LUO
;
Xun LI
;
Linquan ZANG
- Publication Type:Journal Article
- Keywords:
peptide ZS-6;
cDNA library;
subtract biopanning;
lung cancer;
tumor markers
- From:
Chinese Pharmacological Bulletin
2010;26(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Aim To select a potential biomarker for early lung cancer diagnosis and targeted therapy by using the cancer specific bounded peptide ZS-6 which had already been obtained from the laboratory.Methods The peptide ZS-6 marked by biotin was used as a probe to pan out the human lung cancer cDNA phage display library,after 4 rounds of subtraction panning,the specific binding clones of ZS-6 were identified.After amplification and purification,then those DNA sequences were identified and analyzed with bioinformatics.Results 18 phage clones were identified to the specific peptide ZS-6 and the DNA sequence showed one of them was an unknown new gene while the others were known tumor related genes.Conclusion A tumor biomarker selected from human lung cancer cDNA library provides a potential tool for early lung cancer diagnosis and therapy.
