Role of voltage-dependent chloride channels in hippocampal neuronalapoptosis induced by 3-morpholinosydnonimine in rats
10.3867/j.issn.1000-3002.2010.01.002
- VernacularTitle:电压依赖性氯通道在3-吗啉斯德酮亚胺诱导的大鼠海马神经元凋亡中的作用
- Author:
Quanzhong CHANG
;
Shuling ZHANG
;
Jinbao YIN
- Publication Type:Journal Article
- Keywords:
chloride channels;
neurons;
cerebral ischemia;
hippocampus;
patch-clamp techniques,whole cell
- From:
Chinese Journal of Pharmacology and Toxicology
2010;24(1):8-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of chloride channels on the neuronal injury following cerebral ischemia. METHODS Tweleve day in vitro (12dIV) neurons in rats were randomly divided into normal control, 3-morpholinosydnonimine (SIN-1, 1.0 mmol·L~(-1) for 18 h) group, SIN-1+4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid(SITS, 0.5 mmol·L~(-1)) group and SIN-1+4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS, 0.1 mmol·L~(-1)) group. Drugs were added with SIN-1 simultaneously and coincubated for 18 h. The neuronal apoptosis and morphological changes were detected with Hoechst 33258. Chloride channels(ClC)-2/ClC-3 were analyzed with immunofluorescence, the chloride channel currents were recorded with whole cell patch-clamp technique. RESULTS Hoechst 33258 staining showed that the apoptotic percentages were (18.61±0.59) %, (50.43±0.56)%, (23.37±0.52)% and (23.37±0.84)% in normal control group, SIN-1 group, SIN-1+SITS or SIN-1+DIDS groups, respectively. ClC-2/ClC-3 were positively expressed in normal neurons. The currents in neurons exposed to SIN-1 were increased about 55%-56%, SITS and DIDS, two kinds of chloride channel blockers could inhibited the currents about 50%-60% and 30%-40%, respectively. CONCLUSION Voltage-dependent chloride channel maybe participate in the neuronal apoptosis induced by NO, and the activities of chloride channels are perhaps involved in the cerebral ischemic injury.
