Grim-19 overepression induces apoptosis of colon cancer SW480 cells
10.3872/j.issn.1007-385X.2010.01.013
- VernacularTitle:高表达GRIM-19诱导结肠癌SW480细胞凋亡
- Author:
Guihua WANG
;
Xuelai LUO
;
Li SUN
;
Yu DENG
;
Shen WANG
;
Zhaoming LI
;
Xiaolan LI
;
Deding TAO
;
Junbo HU
;
Jianping GONG
- Publication Type:Journal Article
- Keywords:
colon cancer;
GRIM-19;
apoptosis;
Annexin-V/PI;
mitochondrial membrane potential
- From:
Chinese Journal of Cancer Biotherapy
2010;17(1):67-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of retinoid-interferon-induced mortality (GRIM-19) gene on the apoptosis of colon cancer. Methods: A GRIM-19 eukaryotic expression vector (pCMV-Flag-GRIM-19) was constructed and transfected into SW480 cells. Expressions of GRIM-19 and apoptosis-related proteins were detected by Western blotting analysis. Apoptosis of SW480 cells was measured by Annexin-V/PI assay and mitochondrial membrane potential JC-1 staining. Results: The GRIM-19 eukaryotic expression vector pCMV-Flag-GRIM-19 was successfully constructed. Expression of GRIM-19 in SW480 cells was up-regulated and that of apoptosis-related protein Bcl-xl was down-regulated after transfection with pCMV-Flag-GRIM-19. Apoptosis rate was (7.7±1.39)% in SW480 cells transfected with pCMV-Flag empty vector and (15.0 ± 2.52)% in pCMV-Flag-GRIM-19 transfected cells (P<0.05). Mitochondrial membrane potential was decreased in (7.5±2.09)% of pCMV-Flag transfected cells and (17.5±3.07)% of pCMV-Flag-GRIM-19 transfected cells (P<0.05). Conclusion: In vitro GRIM-19 transfection can effectively induce apoptosis of colon cancer SW480 cells.
