Nerve growth factor promotes proliferation of human pancreatic cancer cell line MIA PaCa-2
10.3872/j.issn.1007-385X.2010.01.017
- VernacularTitle:神经生长因子促进MIA PaCa-2人胰腺癌细胞的增殖
- Author:
Lingxin MENG
;
Zhaojun DING
;
Xiping CHEN
;
Hong ZHANG
;
Yuhua CHI
- Publication Type:Journal Article
- Keywords:
pancreatic carcinoma;
nerve growth factor;
proliferation;
cell cycle
- From:
Chinese Journal of Cancer Biotherapy
2010;17(1):88-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of nerve growth factor-β(NGF-β) on the proliferation and cell cycle of human pancreatic cancer MIA PaCa-2 cells. Methods: MIA PaCa-2 cells were treated with different concentrations of NGF-β and K252a (inhibitor of NGF-β receptor TrKA) alone or in combination. Clone forming rate, proliferation, and cell cycle of MIA PaCa-2 cells treated with different strategies were examined by clone formation assay, MTT, and flow cytometry, respectively. Results: NGF-β significantly increased the clone formation and proliferation of MIA PaCa-2 cells (P<0.05, P<0.01). K252a significantly inhibited the proliferation of MIA PaCa-2 cells (P<0.05), while NGF-β combined with K252a had no significant effect on the proliferation of MIA PaCa-2 cells. NGF-β arrested MIA PaCa-2 cell cycle in S phase, K252a arrested cell cycle in G_0/ G_1 phase, and NGF-β combined with K252a arrested cell cycle in S phase. Conclusion: NGF-β can enhance the proliferation of pancreatic carcinoma MIA PaCa-2 cells.
