Effects of resveratrol on proliferation and differentiation of murine 3T3-L1 preadipocytes and the underlying mechanisms
- VernacularTitle:白藜芦醇对小鼠3T3-L1细胞增殖与分化的影响及其机制
- Author:
Sifan CHEN
;
Xincai XIAO
;
Yanshuang SUN
;
Lin ZHENG
;
Xiang FENG
- Publication Type:Journal Article
- Keywords:
resveratrol;
adipocyte;
adiponectin;
leptin;
Sirt1;
PPARγ;
C/EBPα
- From:
Chinese Pharmacological Bulletin
2010;26(1):108-111
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect and mechanism of resveratrol (Res) on proliferation and differentiation of murine 3T3-L1 preadipocytes.Methods 3T3-L1 preadipocytes were cultured and treated with resveratrol in different dosages.Cell proliferation was analyzed by WST-1 method. Oil red O staining method and spectrophotography were applied to analyze the degree of differentiation. Real-time PCR was applied to detect the mRNA expression of adiponectin and leptin. Western blot was applied to detect the expression of silent information regulator 1 (Sirt1),peroxisome proliferator activated receptor γ (PPARγ) and CCTTA enhancer binding proteinα (C/EBPα).Results Res inhibited proliferation of murine 3T3-L1 preadipocytes in a time-dose dependent manner.The expression levels of adiponectin and leptin mRNA were decreased, and Res also inhibited 3T3-L1 preadipocytes to differentiate into mature adipocytes. Res increased the expression levels of Sirt1 and decreased the expression levels of PPARγ and C/EBPα.Conclusions Resveratrol can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes.The underlying mechanisms may include enhancing expression of Sirt1 and inhibiting expression of PPARγ,C/EBPα which are related to cell differentiation.
