Effects of Hydrogen Sulfide on Apoptosis of PC12 Cells Induced by Chemical Hypoxia
- VernacularTitle:硫化氢对化学性缺氧诱导PC12细胞凋亡的影响
- Author:
Jinlan MENG
;
Aiping LAN
;
Ruixian GUO
;
Chuntao YANG
;
Zhanli YANG
;
Xue HUANG
;
Jianqiang FENG
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
cobalt chloride;
hypoxia;
PC12 cells;
apeptosis
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2010;31(1):79-84
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the cytoprotecfion of hydrogen sulfide (H_2S) against cobalt chloride (CoCl_2)-induced apeptosis in PC12 cells and the underlying mechanisms. [Methods] CoCl_2 (a chemical hypoxia-mimetic agent) was used to establish the chemical hypoxia-induced PC12 cell injuries model. Sodium hydrosulfide (NaHS) was used as a H_2S donor. The viability of PC12 cells was measured by CCK-8 assay. The percentage of apeptotic cells was assessed by propidium iodide stain flow cytometry (FCM). The morphological change of apeptotic cells was tested by using the chromatin dye Hoechst 33258. The mitochondrial membrane potential (MMP) was analyzed by rhodamine 123 staining and photofluorography. The level of reactive oxygen species (ROS) in PC12 cells was measured by DCFH-DA staining and photofluorography. [Results] CoCl_2 induced a decrease in cell viability and an increase in percentage of apeptosis in PC12 cells along with dissipation of MMP as well as overproduction of ROS. When PC12 cells were treated with Naris 30 min before CoCl_2 treatment a decrease in viability of PC12 cells induced by 600 μmol/L CoCl_2 was concentration-dependently blocked by NaHs (100, 200, and 400 μmol/L). Pretreatment with NaHS at 200 and 400 μmol/L obviously reduced the apepetotic percentage of PC12 cells induced by 600 μmol/L CoCl_2 and inhibited the dissipation of MMP and overproduction of ROS. [Conclusion] H_2S protected PC12 cells against CoCl_2-induced apeptosis, which may be associated with the inhibition of H_2S on the dissipation of MMP and overproduction of ROS induced by CoCl_2.
