Sensitivity in typeⅠ hypersensitivity compared between BN rats and Wistar rats
10.3867/j.issn.1000-3002.2010.01.006
- VernacularTitle:BN大鼠与Wistar大鼠Ⅰ型超敏反应敏感性的比较
- Author:
Zhonggang LI
;
Huidi QIN
;
Huaishan WANG
;
Yanqiu SHI
;
Zhaoping LIU
- Publication Type:Journal Article
- Keywords:
hypersensitivity,immediate;
models,animal;
immunoglobulin E;
histamine;
tryptases
- From:
Chinese Journal of Pharmacology and Toxicology
2010;24(1):30-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare sensitivity in typeⅠ hypersensitivity between BN and Wistar rats, and to establish a sensitive and reliable determination system for typeⅠ hypersensitivity. METHODS BN and Wistar rats were sc given ovalbumin (OVA) 10, 20 and 40 μg·kg~(-1) every other day for 5 times and normal control group with sc normal saline. The total immunoglobulin E (IgE) levels in serum were determined with ELISA and the specific IgE levels in serum were determined by passive cutaneous anaphylaxis on the 21st day from the 1st injection. The blood pressure, serum histamine and tryptase levels were determined after challenge on the 22nd day. RESULTSTotal IgE, specific IgE, histamine and tryptase levels in serum significantly increased and blood pressure decreased in OVA 10, 20 and 40 μg·kg~(-1) BN rat groups compared with normal control group, while in Wistar rats these symptoms only appeared in OVA 40 μg·kg~(-1) group. CONCLUSION BN rats are more sensitive than Wistar rats in typeⅠ hypersensitivity. The blood pressure, serum total IgE, specific IgE, histamine and tryptase levels can be used as the important indicators in typeⅠ hypersensitivity.
