Change and significance of peripheral blood Th17 cells in patients with acute asthma
10.3969/j.issn.1672-7347.2010.02.007
- VernacularTitle:急性哮喘患者外周血中Th17细胞的变化及其意义
- Author:
Jianmin LI
;
Xudong XIANG
;
Jinxiu LI
;
Wenpu LI
;
Shaokun LIU
- Publication Type:Journal Article
- Keywords:
asthma;
Th17 cell;
interleukin-17;
airway inflammatory
- From:
Journal of Central South University(Medical Sciences)
2010;35(2):129-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of peripheral blood Th17 cells and relationship between the severity and Th17 cells in patients with acute asthma.Methods We recruited patients with mild acute asthma(n=10) and severe acute asthma(n=10), and healthy volunteers(n=10). T-lymphocytes were collected from the peripheral blood mononuclear cells (PBMC). Flow cytometer (FCM) was used to detect the expression of peripheral blood Th17 cells. IL-17 levels in the peripheral blood were measured by enzyme-linked immunosorbentassay (ELISA).Results The rate of positive Th17 cells of peripheral blood in the severe acute asthma group was higher than that in the mild acute asthma group(P<0.05) and the rate of positive Th17 cells of peripheral blood in healthy volunteer group were the lowest among all groups (P<0.05, respectively). The level of IL-17 in the peripheral blood of patients with severe acute asthma increased significantly compared with that in patients with mild acute asthma and healthy volunteers (P<0.05). The positive Th17 cells of peripheral blood in patients with acute asthma were positively correlated with the severity of acute asthma(r=0.869, P<0.05).Conclusion The positive rate of Th17 cells in the peripheral blood increases in patients with acute asthma and has positive correlation with the severity of acute asthma.