Change of HIF-1α protein expression in the placenta bed and concentration of vWF in maternal peripheral blood of pre-eclampsia
10.3969/j.issn.1672-7347.2010.02.008
- VernacularTitle:子痫前期产妇胎盘床HIF-1α蛋白的表达与母体外周血vWF浓度的变化
- Author:
Mei PENG
;
Yiling DING
;
Ling YU
;
Changju ZHOU
- Publication Type:Journal Article
- Keywords:
pre-eclampsia;
placenta bed;
hypoxia-inducible factor 1α;
von Willbrand factor
- From:
Journal of Central South University(Medical Sciences)
2010;35(2):134-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of HIF-1α protein in the placenta bed and the concentration of von Willrand factor (vWF) in maternal peripheral blood from pre-eclampsia and normal pregnancy, and to determine the effect of HIF-1α and vWF on the pathogenesis of pre-eclampsia. Methods Forty pre-eclampsia patients (20 mild and 20 severe) were recruited as 2 study groups, and another 20 normal pregnant women were served as a normal group.Western blot was used to detect the expression of HIF-1α protein in the placenta bed. ELISA was adopted to detect the concentration of vWF in the maternal peripheral blood.Correlation between HIF-1α protein expression and vWF level was analyzed by Spearman method.Results The expression of HIF-1α protein in the placenta bed was the highest in the severe pre-eclampsia patients among 3 groups, followed by the mild pre-eclampsia patients and the normal controls.There was significant difference (among) 3 groups (P＜0.001).The concentration of vWF in the maternal peripheral blood was the highest in the severe pre-eclampsia patients, followed by the mild pre-eclampsia patients and the normal controls.There was significant difference among the 3 groups (P＜0.05). Postive correlation was found between the expression of HIF-1α protein in the placenta bed and the concentration of vWF in the maternal peripheral blood in patients with pre-eclampsia (r_1=(0.65,) P＜(0.001)),and between the concentration of vWF in the maternal peripheral blood and the pathogenetic condition degree of pre-eclampsia (r_2=(0.61,)P＜0.001).Conclusion HIF-1α in coordination with vWF may play an important role in the pathogenesis of pre-eclampsia.
