Development of HBsAg-Binding Aptamers that bind HepG2.2.15 cells via HBV surface antigen
10.1007/s12250-010-3091-7
- Author:
Jia LIU
;
Yan YANG
;
Bin HU
;
Zhiyong MA
;
Hangping HUANG
;
Yuan YU
;
Shenpei LIU
;
Mengji LU
;
Dongliang YANG
- Publication Type:Journal Article
- Keywords:
Aptamer;
Systematic evolution of ligands by exponential enrichment(SELEX);
Hepatitis B virus (HBV);
HBsAg;
Hepatocytes
- From:
Virologica Sinica
2010;25(1):27-35
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus surface antigen(HBsAg),a specific antigen on the membrane of Hepatitis B virus (HBV)-infected cells,provides a perfect target for therapeutic drugs.The development of reagents with high affinity and specificity to the HBsAg is of great significance to the early-stage diagnosis and treatment of HBV infection.Herein,we report the selection of RNA aptamers that can specifically bind to HBsAg protein and HBsAg-positive hepatocytes.One high affinity aptamer,HBs-A22,was isolated from an initial 115 mer library of ～1.1×1015 random-sequence RNA molecules using the SELEX procedure.The selected aptamer HBs-A22 bound specifically to hepatoma cell line HepG2.2.15 that expresses HBsAg but did not bind to HBsAg-devoid HepG2 cells.This is the first reported RNA aptamer which could bind to a HBV specific antigen.This newly isolated aptamer could be modified to deliver imaging,diagnostic,and therapeutic agents targeted at HBV-infected cells.
