Effect of Advanced Glycation End Products on Oxidative Stress and Senescence of Trabecular Meshwork Cells.
10.3341/kjo.2012.26.2.123
- Author:
Cheul Ho PARK
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Advanced glycation end products;
Aging;
Antioxidants;
Oxidative stress;
Trabecular meshwork cells
- MeSH:
Acetylcysteine/metabolism;
Apoptosis/drug effects/physiology;
Arginine/metabolism;
Cell Aging/drug effects/*physiology;
Cell Survival/drug effects/physiology;
Cells, Cultured;
Glycosylation End Products, Advanced/metabolism/*toxicity;
Humans;
Nitric Oxide/metabolism;
Oxidative Stress/*physiology;
Pterins/metabolism;
Reactive Oxygen Species/metabolism;
Serum Albumin, Bovine/metabolism/toxicity;
Trabecular Meshwork/drug effects/*metabolism/*pathology
- From:Korean Journal of Ophthalmology
2012;26(2):123-131
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the effect of advanced glycation end products (AGE) on oxidative stress and cellular senescence in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 10, 50, 100, 200 microg/mL of glycated bovine serum albumin (G-BSA) for 5 days. Also co-exposed were L-arginine, sepiapterin, and antioxidant N-acetylcysteine (NAC). Cellular survival and production of nitric oxide (NO), superoxide, and reactive oxygen species were assessed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay, Griess assay, cytochrome c assay, and dichlorofluorescin diacetate assay, respectively. Senescence-associated beta-galactosidase staining was performed to quantify the degree of cellular senescence. RESULTS: G-BSA decreased cellular survival, NO production, and increased superoxide production significantly in a dose-dependent manner. The effects of G-BSA were abolished with co-exposure of L-arginine, sepiapterin, and NAC. G-BSA enhanced cellular senescence accompanied by increased production of reactive oxygen species. G-BSA-induced cellular senescence was suppressed by application of L-arginine, sepiapterin, and NAC. CONCLUSIONS: AGE enhances cellular senescence of HTMC accompanied with increased oxidative stress. AGE-induced oxidative stress and cellular senescence could be delayed by application of anti-oxidants.
