Injured effect of cerebrospinal fluid from experimental subarachnoid hemorrhage after cerebral lymphatic blockage on PC12 cells
- VernacularTitle:脑淋巴引流阻滞后蛛网膜下腔出血兔脑脊液对PC12细胞的损伤作用
- Author:
Xuan WANG
;
Lili JIA
;
Baoliang SUN
;
Mingfeng YANG
;
Hui YUAN
;
Yanbo ZHANG
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Brain lymphatic drainage;
PC12 cells;
Protein Bax;
Heat-shock protein 70
- From:
Chinese Journal of Pathophysiology
2010;26(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine the injured effect of cerebrospinal fluid(CSF) from subarachnoid hemorrhage(SAH) after cerebral lymphatic blockage(CLB) on PC12 cells. METHODS:SAH and CLB models of adult New Zealand rabbits were used. CSF was obtained from experimental animals after 5 d of modeling and was added into cultured PC12 cells. The cells were randomly divided into blank control(F12 Ham's),normal CSF,SAH CSF,and SAH+CLB CSF groups. At different time points,the survival rate of PC12 cells was measured by MTT assay. LDH leakage was detected. Expression of Bax and heat-shock protein 70(HSP70) was determined by immunohistochemical staining. RESULTS:MTT assay and detection of LDH leakage revealed that the survival rate of PC12 cells was obviously inhibited and the leakage of LDH increased in SAH CSF group and SAH+CLB CSF group. CSF from normal rabbit did not damage the PC12,as compared to blank controls. Above effects were more obvious in SAH+CLB CSF group than those in SAH CSF group. Bax and HSP70 protein expression was found in both SAH CSF group and SAH+CLB CSF group. Expression of Bax protein in SAH+CLB CSF group was stronger than that in SAH CSF group in a time dependent manner. At 0.5 h and 1 h,the expression of HSP70 protein in SAH+CLB CSF group was stronger than that in SAH CSF group,whereas the expression became weaker at 2 h and 4 h in that group. CONCLUSION:Blockage of cerebral lymphatic drainage pathway deteriorates the damage of CSF from SAH on PC12 cells,indicating this pathway may acts as an endogenous protective mechanism in SAH.
