Caspase-3 gene silencing attenuates apoptosis in alveolar epithelial cells after Streptococcus pneumoniae infection
- VernacularTitle:Caspase-3基因沉默对肺炎链球菌感染的肺泡上皮细胞凋亡的影响
- Author:
Mingwang JIA
;
Wenjie HUANG
- Publication Type:Journal Article
- Keywords:
Alveolar epithelial cells;
Apoptosis;
Caspase-3;
Streptococcus pneumoniae;
siRNA
- From:
Chinese Journal of Pathophysiology
2010;26(1):132-136
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the influence of Streptococcus pneumoniae on the apoptosis of alveolar epithelial cell and the modulation of caspases-3 on apoptosis. METHODS: A study was undertaken to determine whether Streptococcus pneumoniae infection induced the apoptosis of A549 and facilitated the expression of pro-inflammative cytokine. After inhibited by siRNA, the expression of caspase-3 and apoptosis grade in A549 were determined. RESULTS: Streptococcus pneumoniae contributed to alveolar epithelial cell apoptosis in a time-dependent manner. Streptococcus pneumoniae facilitated the expression of caspase-3 and IL-6 in A549, while depressed IL-10 concentration in culture medium. SiRNA directly decreased the expression of caspase-3 to rescue A549 from apoptosis but not affected IL-6 and IL-10 concentration. CONCLUSION: caspase-3 gene silencing rescues epithelial cells from apoptosis initiated by Streptococcus pneumoniae infection.
