Effects of sera containing Kang xian ling on c-Met and its downstream MAPK signal molecules in HK-2 cells treated with TGF-β_1
- VernacularTitle:中药抗纤灵方含药血清对TGF-β_1刺激的HK-2细胞c-Met及其下游MAPK信号分子的调控作用
- Author:
Haiying LU
;
Kejian LIU
;
Yue ZHANG
;
Liqun HE
;
Yumin LIU
;
Jing LI
;
Zhifen HAN
;
Huifang PANG
- Publication Type:Journal Article
- Keywords:
Kang xian ling;
Renal tubular epithelial cells;
Transforming growth factor beta;
Hepatocyte growth factors;
MAPK signal pathway
- From:
Chinese Journal of Pathophysiology
2010;26(1):154-157
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of sera containing Kang xian ling (a traditional Chinese medicine) on c-Met and its downstream MAPK signal molecules in HK-2 cells treated with transforming growth factor-β_1(TGF-β_1), so as to further explore the effective mechanism of anti-renal fibrosis of the traditional Chinese medicine. METHODS: Rat sera containing herbs were collected after rats were intragastrically administered with the herbs. HK-2 cells activated by TGF-β_1 were incubated with different heat-inactivated sera. The proliferation and expression of MAPK and the phosphorylation of MAPK in HK-2 cells were detected by Alarm blue and immunoblotting. RESULTS: No significant difference of cell proliferation between any two groups was observed. C-Met and the phosphorylation of its downstream molecules were also observed after incubated with different sera. CONCLUSION: The receptor c-Met of hepatocyte growth factor, and the phosphorylation of its downstream MAPK molecules can be regulated by the sera containing Kang xian ling.
