Effect of VASP mutant phosphorylation on migration of endothelial cells induced by PDGF-BB
- VernacularTitle:血管扩张刺激磷蛋白的磷酸化位点突变对PDGF-BB诱导细胞迁移的影响
- Author:
Yahui ZHANG
;
Deling ZHANG
;
Jingping OUYANG
- Publication Type:Journal Article
- Keywords:
Vasodilator-stimulated phosphoprotein;
Cell movement;
Platelet-derived growth factor
- From:
Chinese Journal of Pathophysiology
2010;26(1):32-36
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of vasodilator-stimulated phosphoprotein (VASP) phosphorylation on the cell migration induced by platelet-derived growth factor BB (PDGF-BB), and to identify which phosphorylation site was more important among the three phosphorylation sites, namely Ser157,Ser239 and Thr278. METHODS: Two phosphorylation mutants of VASP, pcDNA3.1(+)/VASP-S157A and pcDNA3.1(+)/VASP-S239A, were constructed and respectively transfected into the cultured ECV304 cells by means of liposome. The stable expression cells were screened by using antibiotic G418. Protein expression of VASP was measured by Western blotting. The ECV304 cell migration was evaluated using Transwell chamber. RESULTS: Compared to control group, the cell migration was significantly inhibited in ECV304 transfected with VASP-S157A and VASP-S239A (P<0.05), although slight differences existed between VASP-S157A and VASP-S239A transfected cells (P>0.05). CONCLUSION: VASP mutation on the phosphorylation sites of Ser157 and Ser239 inhibits cell migration, and the phosphorylation sites of Ser157 and Ser239 both greatly affect the function of VASP.
