Study on mechanisms of immune suppression mediated by myeloid derived suppressor cells
- VernacularTitle:髓样抑制细胞免疫抑制机理的研究
- Author:
Lin WAN
;
Xin HU
;
Xianxian ZHAO
;
Xiaoyan LI
;
Zhuoya LI
;
Bingjiao YIN
- Publication Type:Journal Article
- Keywords:
MDSC;
NO;
ROS;
IL-10;
TGF-β
- From:
Chinese Journal of Immunology
2010;26(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanisms of immune suppression mediated by Gr-1+CDllb+ myeloid derived suppressor cells (Gr-1~+CDllb~+MDSC)from tumor-bearing mice.Methods:Gr-1~+CDllb~+MDSC recruited into spleen and bone marrow of tumor-bearing mice were purified by Percoll,and suppression mediated by MDSC on T cell proliferation from spleen of naive mice was detected by flow cytometry with CSFE and FTTC-anti CD3 staining,and NO,ROS,IL-10 and TGF-β in the supematant of MDSC were detected by Griess and ELISA.Results:There were much more Gr-1~+CDllb~+MDSCs in spleen and bone marrow from tumor-bearing mouse than those of naive mouse,and suppression on T cell proliferation mediated by MDSC from tumor beating mouse was significantly increased,and there were much more NO,ROS,IL-10 and TGF-βin the supematant of these MDSC than that from naive mouse.Conclusion:MDSC from tumor-bearing mice secreted hish level of NO,ROS,IL-10 and TGF-βto induce immune suppression,and inhibite the proliferation of T cells.
