Prediction of the secondary structure and B cell epitopes for the Izumo protein of Homo Sapiens
- VernacularTitle:人Izumo蛋白的二级结构及B细胞抗原表位预测
- Author:
Xia YANG
;
Kaijun LIU
;
Zigang SHEN
;
Haiyang HE
;
Ji ZHANG
;
Qiaoyu ZHANG
;
Yuzhang WU
;
Jintao LI
- Publication Type:Journal Article
- Keywords:
Izumo protein;
Secondary structure;
B cell epitopes
- From:
Chinese Journal of Immunology
2010;26(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To predict and analyze the secondary structure and B cell epitopes of Izumo protein.Methods: The secondary structure and flexible regions of Izumo protein were predicted by the methods of Chou-Fasman,Gamier-Robson and Karplus-Schulz.Moreover,hydrophilicity plot,surface probability plot and antigenic index of Izumo protein were predicted by the methods of Kyte-Doolitde,Emini and Jameson-Wolf,respectively.Results: Izumo protein contained moreαhelix regions.There were several centers ofαhelix in the regions of 6-17,30-40,88-99,103-120,153-160,173-188,249-260,283-297,334-338 and 339-346 of Izumo protein,and several centers of βsheet in the regions of 21-25,198-200,245-248 and 320-323.Moreover,many distinct B cell epitopes in Izumo protein possibly localized in the regions of 3642,62-66,94-99,118-122,129-132,151-154,161-164,173-177,205-208,212-216,256-265,271-276,283-288,314-318 and 336-350.Conclusion:These results are helpful for identification of the dominant B cell epitopes and the functional domains of Izumo protein.
