The study on non-freezing cold injury induced apoptosis of dorsal root ganglion in the sciatic nerve
- VernacularTitle:坐骨神经非冻结性冷损伤后背根神经节细胞的凋亡
- Author:
Juexian SONG
;
Min XU
;
Zhiwei GENG
;
Jianping JIA
- Publication Type:Journal Article
- Keywords:
Dorsal root ganglion Sciatic nerve Apoptosis Non-freezing cold injury FCM
- From:
Chinese Journal of Nervous and Mental Diseases
2010;36(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the presence of the apoptotic sensory neurons in rat dorsal root ganglion (DRG) following non-freezing cold injury of the sciatic nerve.Methods Thirty three male Wistar rats were randomly divided into three groups (7 d group, 14 d group, 21 d group; n=11 each).The sciatic nerves were cooled to 4 ℃ for 2 hours and the control side underwent sham operation.The pathological examination was performed on L4-6 DRGs at 7, 14, and 21 d post operation.The apoptotic sensory neurons of L4-6 DRGs were detected using FCM (Annexin /PI) and TUNEL staining.Results The apoptotic sensory neurons of L4-6 DRGs were revealed after non-freezing cold injury of the rat sciatic nerve.FCM (Annexin /PI) and TUNEL quantitative analysis indicated that the apoptotic neurons significantly increased in rat dorsal root ganglion (DRG) at 7 d, 14 d, and 21 d post operation following non-freezing cold injury of the sciatic nerve.TUNEL qualitative analysis further confirmed pathological characters of neuron apoptosis in L4-6 DRGs.The apoptotic neurons began to increase from 7~(th) day, reached peak at 14~(th) day, and then decreased slightly at 21 d following operation.Conclusions Non-freezing cold injury on sciatic nerves can cause the sensory neurons apoptosis of L4-6 DRGs.
