Role of heat shock protein 90 in protective effect of hydrogen sulfide against PC12 cells injuries induced by chemical hypoxia
- VernacularTitle:热休克蛋白90在硫化氢保护PC12细胞对抗化学性缺氧损伤中的作用
- Author:
Jinlan MENG
;
Aiping LAN
;
Chuntao YANG
;
Zhanli YANG
;
Liwei WANG
;
Lixin CHEN
;
Linyan ZHU
;
Peixi CHEN
;
Jianqiang FENG
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
cobalt chloride;
chemical hypoxia;
heat shock proteins;
cytoprotection;
apoptosis;
PC12 cells
- From:
Chinese Pharmacological Bulletin
2010;26(1):103-107
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the role of heat shock protein 90(Hsp90)in hydrogen sulfide-induced protection against PC12 cells injuries elicited by cobalt chloride(CoCl_2).Methods Hydrogen sulfide preconditioning against CoCl_2-induced injury model was set up in PC12 cells.Cell viability was tested by using cell counter kit-8.Morphological changes in apoptotic PC12 cells were detected by Hoechst 33258 staining and photofluorography.Apoptotic rate was evaluated by propidium iodide(PI)staining and flow cytometry(FCM).The expression of Hsp90 was evaluated by Western blot.Results Hsp90 expression was upregulated after treatment with 400 μmol·L~(-1) sodium hydrosulfide(a H_2S donor),peaking at 3 h,returning to the basal level at 24 h.Furthermore,H_2S preconditioning obviously enhanced the upregulation of Hsp90 expression induced by CoCl_2.H_2S preconditioning markedly protected PC12 cells against injuries induced by CoCl_2,increasing the viability of cells and decreasing the percentage of apoptotic cells.17-allylamino-17-demethoxygeldanamycin(17-AAG),an inhibitor of Hsp90,antagonized H_2S preconditioning-induced Hsp90 activation and the adaptive cytoprotection.Conclusion H_2S can protect PC12 cells against CoCl_2-induced injuries,and upregulating the expression of Hsp90 may be one of the mechanisms underlying cytoprotection induced by H_2S preconditioning.
