Effects of Porphyromonas gingivalis on focal adhesive components paxillin and FAK of gingival epithelial cells
10.3969/j.issn.1001-3733.2010.01.02
- VernacularTitle:牙龈卟啉单胞菌对牙龈上皮细胞焦点黏附成分paxillin和FAK的作用
- Author:
Yurong KOU
;
Yaping PAN
- Publication Type:Journal Article
- Keywords:
Porphyromonas gingivalis;
Paxillin;
Focal adhesion kinase
- From:
Journal of Practical Stomatology
2010;26(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To explore the destructive mechanism of P.gingivalis on periodontium and to better understand the pathogenic effects of P.gingivalis fimbriae. Methods:Western blotting was used to detect the degradation effects of P.gingivalis ATCC 33277 and its fimA-deficient mutant on focal adhesion components paxillin and focal adhesion kinase (FAK) of Hela cells and immortalized gingival epithelial cells (IHGE cells). Results:P.gingivalis ATCC 33277 wild strain and its fimA-deficient mutant induced degradation of paxillin and FAK both in Hela cells and in IHGE cells. fimA-deficient mutant had a remarkable weaker degradation ability than the wild strain. In IHGE cells, paxillin and FAK were degraded in a time-and MOI-dependent manner. Conclusion:P.gingivalis fimbriae-mediated adhesion and invasion to epithelial cells may promote the degradation of focal adhesion components. IHGE cells may be more suitable for the study of periodontal pathogens than Hela cells.
