An experimental study of folic acid rivaling methylenetetrahydrofolate reductase (MTHFR) gene
10.3969/j.issn.1001-3733.2010.01.03
- VernacularTitle:叶酸拮抗亚甲基四氢叶酸还原酶基因沉默的实验研究
- Author:
Wenlin XIAO
;
Bing SHI
;
Qian ZHENG
;
Yan WANG
;
Lei HUANG
- Publication Type:Journal Article
- Keywords:
Cleft palate;
Embryonic palatal mesenchyme;
Methylenetetrahydrofolate reductase;
RNA interference;
Mouse;
Folic acid
- From:
Journal of Practical Stomatology
2010;26(1):15-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To further understand the role of folic acid supplements rivaling MTHFR gene silencing in pathogenesis of NCLP, RNA interference (RNAi) was applied to knock down MTHFR in mouse embryonic palatal mesenchymal (EPM) cells. Methods: MTHFR ShRNA expression vector were transfected into the primary cultured EPM cells. MTT was used to observe cell proliferation after MTHFR gene silencing. FCM was used to observe cell cycle after MTHFR gene silencing. Results: The results showed the cells proliferation had an inequality amelioration after using folic acid supplements in MEPM cells with MTHFR gene silencing. Using folic acid supplements rivaled the effect of MTHFR gene silencing had a dose-dependent manner. Using 20 μg/ml folic acid supplements could improve the cell proliferation to achieve normal level of cell proliferation. Conclusion: MTHFR gene is an important candidate gene of NCL/P. Using folic acid supplements could prevent teratogenic MTHFR gene silencing for embryonic palate development.
