Evaluation of the biocompatibility of acellular nerve scaffold via three sterilization methods
10.3969/j.issn.1001-3733.2010.01.07
- VernacularTitle:评价三种消毒方法处理脱细胞神经支架的生物相容性
- Author:
Weiqi WANG
;
Dechao LI
;
Wei LU
;
Yongjie ZHANG
;
Yan JIN
- Publication Type:Journal Article
- Keywords:
Acellular nerve scaffold;
Sterilization methods;
Peracetic acid;
MTT;
Tests for local effects after implantation
- From:
Journal of Practical Stomatology
2010;26(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the biocompatibility of acellular nerve scaffold (ANS) via three sterilization methods, to provide experimental data for tissue engineering industrialization. Methods: Pig sciatic nerves were cut and treated using the NaOH maceration method. ANSs were sterilized by ethylene oxide, ~(60)Co-irradiation and peracetic acid. Evaluated the biocompatibility by MTT, cellular compatibility test, collagenase susceptibility test in vitro and local implantation test. Results: ANS retained the integrity of structure and major components of the basement membrane. The result of MTT test showed that the ANSs via different sterilization methods had statistical differences. There were no overall significant differences in Collagenase susceptibility test. Scanning electron microscope results showed the skin fibroblasts could attach, proliferate and grow well on the surface and holes of ANS with sterilization of PAA and Co~(60),a small quantity of cells adhered on ANS with sterilization of ETO. Tests for local effects after implantation show that different sterilization methods don't effect the ability of ANS to resist the enzyme degradation. In ETO group, rats showed an acute inflammatory response followed by chronic inflammation. In PAA and ~(60)Co group rats showed an acute inflammatory response that diminished such that the graft ultimately became indistinguishable from native tissue, observations that were consistent with graft acceptance. Conclusion: Peracetic acid sterilization offers a convenient alternative protocol for ANS processing. ANS sterilized with PAA shows good compatibility and biologic safety. It is an ideal sterilization method for ANS.
