Application of rat tail collagen in the culture of cervical-loop epithelial cells in rat lower incisors
10.3969/j.issn.1001-3733.2010.01.011
- VernacularTitle:鼠尾胶原在培养鼠下切牙颈环上皮细胞中的应用
- Author:
Xiaoli HU
;
Duo WANG
;
Weidong LENG
;
Zhixiao LUO
- Publication Type:Journal Article
- Keywords:
Cervical-loop;
Epithelial cell;
Cell culture;
Rat tail collagen
- From:
Journal of Practical Stomatology
2010;26(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a cell culture model of cervical-loop epithelial cells from Wistar rat lower incisors on culture bottle coated with rat tail collagen. Methods: The effect of self-made rat tail collagen on the culture of cervical-loop epithelial cells was observed. The cells were identified by immunohistochemistry staining. Results: Cervical-loop epithelial cells in Wistar rat lower incisors grew well in self-made rat tail collagen. The cervical-loop epithelial cells exhibited positive expression for integrin-β1 and monoclonal antibody CK in immunohistochemistry staining. Conclusion: The cell culture model of cervical-loop epithelial cells in Wistar rat lower incisors with self-made rat tail collagen can be helpful to research tooth development mechanism.
