An Indirect ELISA of Classical Swine Fever Virus Based on Quadruple Antigenic Epitope Peptide Expressed in E.coli
10.1007/s12250-010-3086-4
- Author:
Guozhen LIN
;
Fuying ZHENG
;
Jizhang ZHOU
;
Xiaoan CAO
;
Xiaowei GONG
;
Guanghua WANG
;
Changqing QIU
- Publication Type:Journal Article
- Keywords:
Antigenic epitope;
Bovine viral diarrhoea virus(BVDV);
Classical swine fever virus(CSFV);
Expression;
Indirect ELISA
- From:
Virologica Sinica
2010;25(1):71-76
- CountryChina
- Language:Chinese
-
Abstract:
In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5% sensitivity and 96.7% specificity compared with the indirect hemagglutination(IHA)test.The inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both ≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed.
