Comparative evaluation of the antioxidant capacity of crocetin and crocin in vivo
- VernacularTitle:西红花苷和西红花酸在小鼠体内抗氧化活性对比研究
- Author:
Yang CHEN
;
Ting YANG
;
Juan HUANG
;
Xi TIAN
;
Can ZHAO
;
Le CAI
;
Lijuan FENG
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
gardenia;
crocetin;
crocin;
in vivo;
antioxidant;
comparative
- From:
Chinese Pharmacological Bulletin
2010;26(2):248-251
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the antioxidant capacity of crocetin and crocin in an in vivo system.Methods Column chromatography was applied to the seperation of crocetin and crocin-1 from gardenia.Crocetin(6.25,12.5 and 25.0 mg·kg~(-1)·d~(-1)) and crocin (18.7,37.5 and 75.0 mg·kg~(-1)·d~(-1)) were orally administered to kunming mice.Then,superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),total antioxidant capacity(TAOC)and malondialdehyde(MDA)in mice were determined for the comparison of antioxidant activity of crocetin and crocin-1.Results Oral administration of crocetin and crocin for six weeks could enhance SOD of liver and kidney,GSH-Px of liver and TAOC of heart and kidney.In addition,it could decrease MDA of serum in mice.Conclusions The comparison of results suggests the evidence supporting the comparable antioxidant activity of crocetin and crocin.The results of the research also indicate that liver and kidney are two organs targeted for protection concerning endogenous antioxidant among various tissues.
