Protective effect of total flavonoids from Sorbus TianschanicaL leaf against myocardial ischemia/reperfusion injury in rats
- VernacularTitle:天山花楸叶总黄酮对大鼠心肌缺血/再灌注损伤的保护作用
- Author:
Wei FU
;
Ting LIU
;
Caiyu YANG
;
Ting WANG
;
Qiusheng ZHENG
;
Hui TANG
;
Zhenhua WANG
- Publication Type:Journal Article
- Keywords:
Sorbus Tianschanica L;
total flavonoids;
rats;
myocardial;
ischemia/reperfusion injury;
antioxidative
- From:
Chinese Pharmacological Bulletin
2010;26(2):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect and the underline mechanisms of total flavonoids from Sorbus Tianschanica L leaf (TFST) against myocardial ischemia/reperfusion (I/R) injury.Methods The I/R injury model of rat isolated heart was prepared by improved Langendorff retrograde perfusion method.Following the treatment,the coronary blood flow levels (CF),left ventricular developed pressure (LVDP) and maximal rise/fall rate of left ventricular pressure (±dp/dt_(max)) were monitored as the myocardial function.The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in myocardial tissue were measured by the commercial kits. The in vitro anti-oxidative capacity of TFST was detected by the DPPH and lipid peroxidation reaction system.Results Compared with I/R injury group, pre-incubation with TFST (6.0 mg·L~(-1)) significantly improved the LVDP,±dp/dt_(max) and CF during reperfusion. TFST (6.0 mg·L~(-1)) treatment significantly increased the SOD activities and reduced the MDA levels in myocardium tissue.Moreover,TFST (from 6.25 to 100.0 mg·L~(-1)) scavenged the DPPH, hydroxyl radical and superoxide anion free radicals, and inhibited the lipid peroxidation reaction in a concentration-dependent way.Conclusions All the results demonstrate that TFST possesses the cardioprotective effect on ischemia/reperfusion injury.This efficacy may be due to its antioxidative activity.
