Protective effect of active component of Eclipta on hepatic injury induced by ConA in mice
- VernacularTitle:墨旱莲活性成分对刀豆蛋白A诱导的小鼠肝损伤的作用
- Author:
Ruming XU
;
Kemin DENG
;
Yang LU
- Publication Type:Journal Article
- Keywords:
Eclipta;
Wedelolactone;
Concanavalin A;
liver protection;
anti-inflammatory effect
- From:
Journal of Shanghai Jiaotong University(medical Science)
2010;30(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objecave To explore the protective effect of active component of Eclipta on hepatic injury induced by Concanavalin A(ConA)in mice. Methods Mouse hepatic injury models were established by injection of ConA(15 mg/kg)via tail vein.The effect of Eclipta extracts on serum alanine aminotransferase (ALT) and liver histology in both normal mice and hepatic injury mice was examined.A pure compound was obtained by means of bioactivity-guided isolation,and IR,~(13)C-NMR, ~1H-NMR,~1H-~(13)C HMBC and ~1H-~(13)C HMQC were used for structural identiffcation. Results Eclipta extracts decreased serum ALT in hepatic injury mice induced by ConA.and demonstrated anti-apoptosis effect induced by ConA in hepatoeytes.Wedelolaetone,a eoumarin,was isolated from Eclipta,and its structure was identified on the basis of spectroscopic analysis.Wedelolactone inhibited ConA-induced T cell proliferation selectively,and completely antagonized the effect of ConA at the concentration of 10μg/mL. Conclusion Eclipta herb has hepatoprotective effect.Wedelolactone,the anti-inflammatory and anti-apoptosis active component of Eclipta,might serve as a lead compound for developing new antiinflammatory drugs.
