Protective effect of Tribulus terrestris saponin monomer B on cadiocytes impaired by hypoxia-reoxygenation
- VernacularTitle:蒺藜皂苷单体B对心肌细胞缺氧/复氧损伤的保护作用
- Author:
Shuang ZHANG
;
Hong LI
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
Tribulus terrestris saponin monomer B;
cadiocytes;
hypoxia-reoxygenation;
apoptosis;
caspase-3;
oxygen free radical
- From:
Chinese Pharmacological Bulletin
2010;26(2):208-212
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the protection effect of Tribulus terrestris saponin monomer B (TTSMB) on cadiocytes impaired by hypoxia-reoxygenation (H/R).Methods Cadiocytes of neonate rat were cultivated for 72 hours and divided into normal control group, H/R group,GSTT 100 mg·L~(-1) group and TTSMB 10,1,0.1 nmol·L~(-1) group.Morphocytology change of cadiocytes was observed after the treatment.Cadiocyte survival rate was detected with MTT colorimetric method.Levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), malondialdehyde (MDA), and activity of superoxide dismutase (SOD) were determined. Apoptosis rate was detected with flow cytometry.Expression of caspase-3 was examined with western blot.Results Compared with the control group, the survival cell number in H/R group decreased obviously, content of CK,LDH, AST, MAD increased, and activity of SOD decreased (P<0.01).Compared with the model group, the survival cell population increased in TTSMB 10,1,0.1 nmol·L~(-1) group (P<0.05 and P<0.01).Contents of CK, LDH, AST, MAD decreased, whereas the activity of SOD increased (P<0.05 and P<0.01).Apoptosis rate and expression of caspase-3 also reduced in TTSMB 10,1,0.1 nmol·L~(-1) group.Conclusion TTSMB has significant cadiocytes protective effect against H/R and inhibits cadiocyte apoptosis,and the mechanism depends on the effect against oxygen free radical.
