Effects of Melittin on the growth and G_2/M phase arrest in SGC-7901 cells
- VernacularTitle:蜂毒素对SGC-7901细胞生长及G_2/M期阻滞的影响
- Author:
Baoming WU
;
Jun LI
;
Xiongwen Lü
;
Yong JIN
;
Bin ZHAO
;
Yan HUANG
;
Yiping WANG
;
Rong LOU
;
Pengjing WANG
- Publication Type:Journal Article
- Keywords:
melittin;
SGC-7901 cell;
proliferation;
cell cycle arrest;
CylinB1;
CDK1;
CDc25c
- From:
Chinese Pharmacological Bulletin
2010;26(2):222-225
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of the Melittin on growth and cell cycle of SGC-7901 cells.Methods Growth inhibition effect of Melittin was evaluated using SRB in SGC-7901 cells in vitro;Melittin induced cell cycle arrest was investigated using flow cytometry assay;reverse transcription PCR(RT-PCR)was used to detect the associated protein mRNA of cell cycle.Results Proliferation activity of SGC-7901 cells was inhibited after treatment with Melittin(1,2,4,8,16,32×10~(-3) μg·L~(-1))(P<0.05 or P<0.01)for 24 h;Flowcytometry analysis revealed that SGC-7901 cells accumulated in the G_2/M phase after treatment with Melittin(4,8×10~(-3) μg·L~(-1))for 24 h;the expression of CylinB1,CDK1 and Cdc25c mRNA were decreased.Conclutions Proliferation activity of SGC-7901 cells was inhibited by Melittin,which may be related to the inhibitory effect of Melittin on associated protein transcription in the G_2/M stage of SGC-7901 cells.
