Biological activity of the cultured rabbit bone marrow-derived mesenchymal stem cells in vitro
- VernacularTitle:兔骨髓基质干细胞的体外培养及生物学活性研究
- Author:
Chen ZHANG
;
Kunzheng WANG
;
Hui QIANG
;
Zhibin SHI
;
Xiaoqian DANG
;
Xianghui HUANG
- Publication Type:Journal Article
- Keywords:
bone marrow-derived mesenchymal stem cell;
cell culture;
multi-directional differentiation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(2):138-142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of the in vitro isolation, culture and identification of rabbit bone marrow-derived mesenchymal stem cells (BMSCs) and explore the differentiation potentials of BMSCs. Methods BMSCs were isolated and purified by density gradient centrifugation combined with attachment culture method. The biological characteristics of BMSCs were observed under an inverted microscope. The BMSCs were identified with HE staining and CD34/CD44 immunohistochemical staining. The biological activity of BMSCs was detected by osteogenic induction and steatogenic induction. Results BMSCs were isolated successfully in vitro and had good homogenicity. Immunohistochemical staining of CD34/CD44 showed strong positive expression of CD44 and negative expression of CD34. Fortis osteogenic ability and positive staining of ALP could be detected by osteogenic induction, and fortis steatogenic ability and positive staining of axungia could be detected by steatogenic induction. Conclusion BMSCs have the capability of multi-directional differentiation in vitro.
