Bioinformatical analysis of the specific allergen pTSX1 cDNA clone of Humulus pollen in allergic asthma
- VernacularTitle:致过敏性哮喘的葎草花粉特异性变应原pTSX1 cDNA克隆的生物信息学分析
- Author:
Jing XU
;
Xiuzhen SUN
;
Yun LIU
;
Yonghong ZHANG
;
Wei LI
- Publication Type:Journal Article
- Keywords:
Humulus pollen;
allergen cDNA;
bioinformatics;
sequence analysis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2010;31(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To make a bioinformatical analysis of the specific allergen pTSX1 cDNA clone obtained from Humulus pollen in allergic asthma by immunological screening and gene cloning. Methods Bioinformatical approaches, including sequence analysis, sequence alignment, genetic makeup, protein structure and function prediction, were used. Results The analysis of the specific allergen pTSX1 cDNA showed that the gene had no reading frame and coded a protein with 204 amine acids. The analysis of amine acids sequence coded by the pTSX1 cDNA showed that the protein identity of this gene product might be one of ribosomal proteins. Conclusion The pTSX1 cDNA cloned from Humulus pollen in allergic asthma is a new gene, and the protein coded by pTSX1 gene may be one of ribosomal proteins.
