Knockdown of Moesin Expression Accelerates Cellular Senescence of Human Dermal Microvascular Endothelial Cells.
10.3349/ymj.2010.51.3.438
- Author:
Ju Hee LEE
1
;
Jung Hoan YOO
;
Sang Ho OH
;
Kyu Yeop LEE
;
Kwang Hoon LEE
Author Information
1. Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. kwanglee@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Aging;
endothelial cell;
shRNA lentivirus;
moesin;
p16
- MeSH:
Aged, 80 and over;
Antigens, CD31/metabolism;
Blotting, Western;
Cell Aging/genetics/*physiology;
Cell Line;
Child;
Endothelial Cells/*cytology/*metabolism;
Humans;
Immunohistochemistry;
Microfilament Proteins/genetics/*physiology;
Microscopy, Phase-Contrast;
Microvessels/*cytology;
RNA, Small Interfering/genetics/physiology;
Reverse Transcriptase Polymerase Chain Reaction;
Skin/*blood supply
- From:Yonsei Medical Journal
2010;51(3):438-447
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Endothelial cells maintain the homeostasis of blood, which consists of plasma and cellular components, and regulate the interaction between blood and the surrounding tissues. They also have essential roles in vascular permeability, the circulation, coagulation, inflammation, wound healing, and tissue growth. The senescence of endothelial cells is closely related to the aging of the adjacent tissues and to age-related vascular disease. Recently, the expression of moesin was found to be decreased in elderly human dermal microvascular endothelial cells (HDMECs), and an association between moesin and senescence has been suggested. This study examined the functional role of moesin in cellular senescence. MATERIALS AND METHODS: To study the effects of decreased moesin expression on cellular senescence and metabolism, HDMECs were transfected with short hairpin-RNA (shRNA) lentivirus to silence moesin gene expression. In addition, specimens from young and old human skin were stained with anti-moesin and anti-p16 antibodies as an in vivo study. RESULTS: Using shRNAl-entivirus, moesin knock-down HDMECs developed characteristics associated with aging and expressed senescence associated-beta-galactosidase during early passages. They also showed increased p16 expression, decreased metabolic activity, and cell growth retardation. Human skin tissue from elderly persons showed decreased moesin expression and increased p16 expression. CONCLUSION: These findings suggest that there is a functional association between moesin expression and cellular senescence. Further study of the functional mechanism of moesin in the cytoskeleton and cellular senescence is needed. In addition, this study provides a useful model for developing anti-aging treatments.
