Alteration of AQP2 expression in kidney tissues of emphysema model rats
- VernacularTitle:肺气肿模型大鼠肾组织水通道蛋白2的表达及机制研究
- Author:
Zhe WANG
;
Chunying LIU
;
Yuan GAO
;
Ying WANG
;
Huan JING
;
Jiyan CHAI
;
Shouyan WANG
;
Deshan WANG
- Publication Type:Journal Article
- Keywords:
Pulmonary emphysema;
Aquaporin 2;
Endothelins;
Interleukin-1;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
2010;26(2):318-321
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the expressions of aquaporin 2 (AQP2) in kidney tissues and the contents of endotoxin (ET), interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α) in serum in emphysema model rats, and to investigate the relationship between lungs and kidney in humoral metabolism. METHODS: The rats of emphysema were treated by injecting lipopolysaccharide into the trachea with cigarette smoking. Immunohistochemistry and Western blotting analysis were used to observe the expression of AQP2 in kidney tissues. RT-PCR was applied to detect the expression of AQP2 mRNA in kidney tissues. Blood sample and lung tissue were taken and the levels of ET, IL-1β and TNF-α were measured by radioimmunoassay. RESULTS: AQP2 expression in the kidney tissue in model group was greater than that in control group, and the expression of AQP2 mRNA showed the same results (P<0.01). ET, IL-1β and TNF-α levels in serum and lung tissue in model group were markedly higher than those in control group (P<0.01). CONCLUSION: In the emphysema model rats, AQP2 expression is up-regulated in the kidney tissue. The mechanism of emphysema may be related to increasing the levels of ET, IL-1β and TNF-α in the serum and lung tissue obviously.
