Effect of pressure phase plane derived τ and K on evaluation of left ventricular diastolic function in isolated rat heart during ischemia/reperfusion
- VernacularTitle:基于压力相平面推导的τ和K在评价离体缺血/再灌注大鼠心脏左心室舒张功能中的作用
- Author:
Ying OU
;
Mingzhi ZHENG
;
Jianping JIANG
;
Jiali BAO
;
Yingying CHEN
;
Yueliang SHEN
- Publication Type:Journal Article
- Keywords:
Ischemia/reperfusion injury;
Left ventricular diastolic function;
Pressure phase plane;
Time constant of ventricular isovolumic relaxation;
Chamber stiffness
- From:
Chinese Journal of Pathophysiology
2010;26(2):216-221
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze and compare the changes of pressure phase plane(PPP) derived τ and K on isolated rat heart during ischemia/reperfusion, and to explore the value of PPP derived τ and K for evaluation of left ventricular diastolic dysfunction. METHODS: LVEDP, -d(p/dt)_(max), τ and K were measured and calculated during ischemia/reperfusion in Sprague-Dawley rat hearts. Meanwhile, the level of lactate dehydrogenase (LDH) in the coronary effluent was measured, and the ultrastructure changes in myocardium were observed under electron microscope. RESULTS: Compared with control group, τ increased and K reduced significantly in each ischemic group in a time dependent manner (P<0.05). With prolonged ischemia, τ was even higher and K was even lower (P<0.05). Compared with control group, except ischemia 15 min, LDH in other groups increased significantly at 10 min and 20 min after reperfusion (P<0.05). Compared with ischemia 30 min, LDH of ischemia 45 min and ischemia 60 min were even higher at 10 min and 20 min after reperfusion (P<0.05). With prolonged ischemia, the abnormal changes of the myocardial ultrastructure were observed. CONCLUSION: PPP derived τ and K may be promising indexes for quantitative assessment of left ventricular diastolic function on isolated) rat heart during ischemia/reperfusion, and indication of the severity of ischemia/reperfusion injury.
