Constitutive expression of HIF-1α in BMSCs reduces neuronal apoptosis and promotes neuronal proliferation after stroke in rats
- VernacularTitle:慢病毒载体介导HIF-1α基因修饰骨髓间质干细胞促进脑缺血大鼠的神经功能恢复
- Author:
Weibiao YE
;
Yubin DENG
;
Meihong YE
;
Tuo CAI
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Brain ischemia;
Hypoxia-inducible factor 1;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2010;26(2):256-261
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explored the potential role of HIF-1α in reducing the neuronal apoptosis and promoting the neuronal proliferation after stroke in rats. METHODS: The bone marrow-derived mesenchymal stem cells (BMSCs) were lentivirally transduced to express the stable form of HIF-1α. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in Sprague-Dawley rats. Neurological function was evaluated by modified neurological severity score (mNSS). Cerebral infarct volume was measured by TTC staining. Immunohistochemistry and terminal deoxynucleotidyltransferase mediated dUTP nick end labeling (TUNEL) method were performed to detect neuronal proliferation and apoptosis. RESULTS: Significant improvement of neurological deficits was found in BMSCs-mHIF-1α rats as compared to the control animals at 14th d and 28th d after MCAO (P<0.05). Significant reduction of infarct volume was observed in rats in BMSCs-mHIF-1α group at 3rd day after MCAO (P<0.05). Histological evaluation showed that BMSCs-mHIF-1α treatment significantly promoted neuronal survival and proliferation in the ischemic boundary area. CONCLUSION: Constitutive expression of HIF-1α in BMSCs reduces the neuronal apoptosis and promotes the neuronal proliferation after stroke in rats.
