iASPP on apoptosis in breast cancer cells which expressed wild type p53
- VernacularTitle:iASPP对表达野生型p53基因的乳腺癌细胞株MCF-7凋亡的影响
- Author:
Changsong WANG
;
Hong LI
;
Chunfang GAO
;
Yanping CHEN
- Publication Type:Journal Article
- Keywords:
Protein iASPP;
MCF-7 cells;
Genes;
p53;
RNA interference
- From:
Chinese Journal of Pathophysiology
2010;26(2):282-286
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the RNAi effect of the inhibitory member of the ASPP family (iASPP) on the apoptosis of human breast cancer cell MCF-7 which expressed the wild type p53 gene. METHODS: The recombinant plasmid pAd-iASPP-RNAi was transfected into MCF-7 cells. The expression of iASPP mRNA and protein was analyzed by RT-PCR and Western blotting, respectively. The cell apoptosis was detected by FCM, and then the MCF-7 cells were transplanted into nude mice to set up transplantation model. The expression of iASPP RNA and protein in transplanted neoplasm were determined by RT-PCR and Western blotting, the apoptosis index was detected by FCM at the same time. RESULTS: The results showed that the expression of iASPP descended in MCF-7 cells (mRNA 95.4% and protein 96.8%, respectively, P<0.01) and the apoptosis rate and necrosis rate of MCF-7 cells increased (P<0.01) after transfection. As treated with pAd-iASPP-RNAi, the expression of iASPP in transplantation tumor cells descended 87.4% (mRNA) and 89.2% (protein), respectively (P<0.01), and the apoptosis rate and necrosis rate increased accordingly (P<0.01, P<0.05). CONCLUSION: The inhibition of iASPP may resume the ability of p53 to induce apoptosis in breast cancer cells which is able to express wild type p53.
